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. 2023 Feb 18;24(4):4131. doi: 10.3390/ijms24044131

Figure 4.

Figure 4

(A) The effect of the supplement (SUPPL, 1.2 µM SPD + 92 µM EUG) alone or in combination with ng/mL lipopolysaccharide (LPS) for 2 h on fluorescent green (FG)-stained LC3-II puncta, chromogen red-stained LC3-II and chromogen red-stained P62 in comparison to the untreated control and LPS alone (magnification ×60). All treatments were performed in the presence and absence of 20 µM hydroxychloroquine (HCQ). (B) Quantification of the LC3-II steady state and turnover levels. (C) Quantification of the P62 steady state and turnover levels. Significant differences were represented as follows: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** 0.0001 < p < 0.001, **** p < 0.0001. The black dots indicate the positioning of the individual replicates within the bar for each sample. DAPI = nuclear staining in FG imaging: H = hematoxylin.