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. 2023 Feb 18;24(4):4131. doi: 10.3390/ijms24044131

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Expression (A) of 20 individual human autophagy proteins in NCM460 cells, identified according to the (B) position location on the micro-array. Protein expression (A) was performed in the untreated control (CTRL) and lipopolysaccharide (LPS)-treated cells (1 ng/mL LPS) after 24 h and compared to cells pre-incubated for 1 h with 1.2 µM spermidine (SPD) + 0.2 mM eugenol (EUG) and in supplement (SUPPL) form (1.2 µM SPD + 92 µM EUG) prior to LPS addition. (C) Protein expression for each of the 20 proteins was quantified for each treatment. Significant differences were represented as follows: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** 0.0001 < p < 0.001, **** p < 0.0001. The black dots indicate the positioning of the individual replicates within the bar for each sample.

Expression (A) of 20 individual human autophagy proteins in NCM460 cells, identified according to the (B) position location on the micro-array. Protein expression (A) was performed in the untreated control (CTRL) and lipopolysaccharide (LPS)-treated cells (1 ng/mL LPS) after 24 h and compared to cells pre-incubated for 1 h with 1.2 µM spermidine (SPD) + 0.2 mM eugenol (EUG) and in supplement (SUPPL) form (1.2 µM SPD + 92 µM EUG) prior to LPS addition. (C) Protein expression for each of the 20 proteins was quantified for each treatment. Significant differences were represented as follows: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** 0.0001 < p < 0.001, **** p < 0.0001. The black dots indicate the positioning of the individual replicates within the bar for each sample.