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. 2023 Feb 18;24(4):4131. doi: 10.3390/ijms24044131

Figure 6.

Figure 6

The effect of a pretreatment (1 h) of 1.2 µM spermidine (SPD) and 0.2 mM eugenol (EUG) alone, in combination (1.2 µM SPD + 0.2 mM EUG), and in supplement (SUPPL) form (1.2 µM SPD + 92 µM EUG) prior to the addition of 1 ng/mL lipopolysaccharide (LPS) for 24 h on (A) cell viability, (B) Midkine levels, and (C) ROS levels in NCM460 cells compared to the untreated control (CTRL) and LPS treatment alone. Significant differences were represented as follows: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** 0.0001 < p < 0.001, **** p < 0.0001. The black dots indicate the positioning of the individual replicates within the bar for each sample.