Figure 8.

(A) Exposure of NCM460 cells to the supplement (SUPPL, 1.2 µM spermidine (SPD)) + 92 µM eugenol (EUG), lipopolysaccharide (LPS, 1 ng/mL) and Compound C (CC, 5 µM) either alone or in combination compared to the untreated control (CTRL). (A) Red chromogen-stained LC3-II expression at ×40 mag with (B) LC3-II expression quantification and (C) comparison to midkine levels. Compound C was administered 30 min prior to the addition of the SUPPL and SUPPL pretreatments were for 1 h prior to LPS exposure for 4 h. Significant differences were represented as follows: * 0.01 < p < 0.05, ** 0.001 < p < 0.01, *** 0.0001 < p < 0.001, **** p < 0.0001. The black dots indicate the positioning of the individual replicates within the bar for each sample.