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. 2001 Jun;183(11):3428–3435. doi: 10.1128/JB.183.11.3428-3435.2001

FIG. 3.

FIG. 3

Effect of temperature on the specific activity of the PGI purified from P. furiosus. (A) Temperature dependence of the specific activity. (B) Arrhenius plot of the same data. Enzyme activity was measured in the direction of glucose-6-phosphate formation using either the continuous assay for temperatures below 50°C (▵) or the discontinuous assay system for temperatures above 50°C (●) (see Materials and Methods). The assay mixture contained 0.35 μg of enzyme.