FIG. 6.

Multiple sequence alignment of the PGI superfamily of bacteria and eucarya. Deduced amino acid sequences of B. stearothermophilus B (47), E. coli (14), pig (7), rabbit (20), and human (12) are aligned. In addition, the amino acid sequence of the hypothetical PGI of the archaeon M. jannaschii (MJ1605) is included. The two PGI signature patterns [DENS]-X-[LIVM]-G-G-R-[FY]-S-[LIVMT]-X-[STA]-[PSAC]-[LIVMA]-G- and [GS]-X-[LIVM]-[LIVMFYW]-XXXX-[FY]-[DN]-Q-X-G-V-E-X-X-K are highlighted by boxes. Amino acids that constitute putative substrate-binding sites in accordance with the crystal structure of B. stearothermophilus PGI (45) are shaded.