TABLE 1.

Purification of PGI from P. furiosus^a

Purification step	Protein (mg)	Activity (U)	Sp act (U/mg)	Yield (%)	Purification (fold)
Cell extract	1,118	78.3	0.07	100	1
Q Sepharose	176	65.3	0.37	83	5
Phenyl-Sepharose	9.9	38.6	3.9	49	56
Superdex 200	1.8	31.2	17.7	34	253
Uno Q1	0.7	25.8	34.8	33	497

^aEnzyme activity was measured in a continuous assay at 50°C in the direction of glucose-6-phosphate formation (see Materials and Methods).