TABLE 3.
Molecular properties of glucose-6-phosphate isomerases from bacteria, eucarya, and the archaeon P. furiosus
| Organism | Apparent molecular mass (kDa)
|
Reference(s) | |
|---|---|---|---|
| Native enzyme | Subunite | ||
| Archaea | |||
| P. furiosus | 41a (α2)c | 23.3* | This work |
| Bacteria | |||
| B. stearothermophilus (A, B) | 172b (α4) | 50.3*, 50.1* | 28, 47 |
| B. caldotenax | 202a (α4) | 50.6 | 46 |
| E. coli | 120a (α2) | 61.5* | 14, 39 |
| Lactobacillus casei | 67a (α2) | 34 | 30 |
| Eucarya | |||
| Saccharomyces cerevisiae | 119b (α2) | 61.2* | 25, 48 |
| Aspergillus niger | 118a (α2) | 60 | 32 |
| Pig | 132b (α2) | 63.0* | 1, 7, 9 |
| Mouse | 120a (α2) | 62.6* | 8, 16 |
| Rabbit | 125a (α2) | 64* | 24 |
| Human | NDd (α2) | 63.0* | 12, 49 |
a
Determined by gel filtration.
b
Determined by analytical ultracentrifugation.
c
The proposed subunit composition of the native enzyme is shown in parentheses.
d
ND, not determined.
e
Apparent molecular masses determined by SDS-PAGE. Asterisks (*) indicate molecular masses calculated from amino acid sequences.