FIG. 1.
Schematic representation of the vanD gene cluster in BM4339. (A) Map of the 8.7-kb region containing the vanRD, vanSD, vanYD, vanHD, vanD, vanXD, and intD ORFs comprising the vanD gene cluster. Open arrows indicate sense of transcription. The PCR fragment internal to vanXD used as a probe in hybridization experiments is indicated. Abbreviations: H, HindIII; S, Sau3AI; X, XmaI. (B) Inserts in recombinant plasmids. The inserts are represented by solid lines, and the vectors are indicated in parentheses. (C) Probes used in Northern hybridization and in RT-PCR. (D) Oligodeoxynucleotides used in RT-PCR and in primer extension. Arrowheads indicate positions and orientations of primers.