FIG. 8.
CAT activity in cytoplasmic extracts from E. faecalis JH2-2 and from E. faecium BM4339 harboring plasmid pAT666 (PYD cat). Controls were performed without addition of vancomycin to the culture medium (“not induced” bars), and induction was achieved by adding 1 μg of vancomycin/ml to cultures of JH2-2/pAT666 and 8 μg of vancomycin/ml to cultures of BM4339/pAT666 (“induced” bars). Enzymatic activity was expressed as nanomoles of product formed per minute per milligram of protein in S100 extracts. Results are means ± standard deviations obtained from three independent extracts.