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. 2023 Feb 13;24(4):3709. doi: 10.3390/ijms24043709

Figure 3.

Figure 3

Desferrioxamine inhibits activation of LRRK2 and cell damage caused by 6-OHDA. SH-SY5Y cells were treated with 0, 10, 20, 40, or 60 μM of DFO for 24 h. (A) The cell viability was analyzed by the MTT assay (n = 10). (B) Effect of DFO on the survival of SH-SY5Y cells by LDH assay. SH-SY5Y cells were incubated with 0, 10, 20, or 40 μM DFO for 24 h in the presence of 100 μM 6-OHDA. (C) The level of pS935-LRRK2. (D) The level of pS1292-LRRK2. SH-SY5Y cells were incubated with 0, 10, or 40 μM DFO for 24 h in the presence of 100 μM 6-OHDA. Upper: representative Western blot images of pS935-LRRK2 or pS1292-LRRK2, lower: quantification of the levels of pS935-LRRK2 or pS1292-LRRK2 in SH-SY5Y cells (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001.