TABLE 5.
Strain | Genotypea | Growth rate (h−1)b
|
Sp act of phosphofructokinasec
|
||
---|---|---|---|---|---|
−Erm | +Erm | U/OD600 | % of wt | ||
MG1363 | wt | 0.74 | 5.56 | 100 | |
HWA254 | wt [pfk] | 0.71 | 0.61 | 52.84 | 950 |
HWA217 | wt las::CP25 | 0.46 | 2.18 | 39 | |
HWA256 | wt las::CP25 [pfk] | 0.70 | 0.59 | 33.97 | 611 |
HWA232 | wt las::CP29 | 0.56 | 3.34 | 60 | |
HWA258 | wt las::CP29 [pfk] | 0.71 | 0.55 | 50.65 | 911 |
All mutant strains are MG1363 derivatives. The synthetic promoters transcribing the las genes are shown. The strains in which pfk is overexpressed from pMU2916 carrying pfk are indicated. wt, wild type.
Specific growth rates are shown for cultures growing in the absence (−) or presence (+) of erythromycin.
Specific activities (U/OD600), in micromoles per minute per cell density of phosphofructokinase are shown for culture growth in the presence of erythromycin. The activities from each strain were determined in triplicate. The specific activities of phosphofructokinase are percentages of that for MG1363.