TABLE 7.
Glycolytic flux in resting cells of las mutants with reduced phosphofructokinase activity
| Strain | Genotypea | Relative phosphofructokinase activity (%) | Rate of glucose consumption (mmol · g [dry wt]−1 · h−1)b | Rate of lactate production (mmol · g [dry wt]−1 · h−1)b |
|---|---|---|---|---|
| MG1363 | wt | 100 | 15.1 | 27.1 |
| HWA217 | wt las::CP25 | 39 | 14.5 | 25.9 |
| HWA232 | wt las::CP29 | 60 | 14.3 | 24.5 |
a
wt, wild type.
b
Rates of glucose consumption and lactate production were determined from the slopes of glucose and lacate concentration versus time and normalized by the cell dry weight. The correlation coefficients for all experiments ranged from 0.980 to 0.986.