FIG. 1.

Comparison of [³⁵S]methionine-labeled FhuA proteins and FhuA barrel-cork hybrids after transformation of E. coli CH21 with plasmid pHK763 (lane 1), pEcBSpC (lane 2), pEcBStC (lane 3), pEcBPaC (lane 4), pBK7 (lane 5), p76Sp (lane 6), pSpBEcC (lane 7), pSpBStC (lane 8), pSpBPaC (lane 9), pSpΔ5–160 (lane 10), p76St (lane 11), pStBEcC (lane 12), pStBSpC (lane 13), pStBPaC (lane 14), pStΔ5–160 (lane 15), p76Pa (lane 16), pPaBEcC (lane 17), pPaBSpC (lane 18), pPaBStC (lane 19), or pPaΔ5–160 (lane 20). No other bands were seen outside the gel section represented here. Nomenclature used: EcBSpC means the β-barrel (B) of E. coli FhuA and cork (C) of S. paratyphi B FhuA; the other designations follow the same rule. Ec, E. coli; Sp, S. paratyphi B; St, Salmonella serovar Typhimurium; Pa, P. agglomerans.