Synchrotron microbeam X-ray fluorescence mapping of hair cross-sections originating from different body parts (scalp regions, eyebrow, underarm, pubic, and leg hair) donated by the same person sectioned with a microtome to a thickness of 10 µm (light micrographs of hair cross sections). The elemental distribution of the detected elements is shown across the different hair morphological regions. Elemental maps were, all 80 µm × 80 µm in size, produced with a 500 nm X-ray beam spot size to compromise between the signal and size of the features of interest. The detected elements increase from the blue color to the red color in the scale bar, with the colors indicating relative element content.