Table 1.
Primers and probes that were employed for this study.
| Primers and Probes | Sequences (5′-3′) |
Length (Base Pair) |
Use |
|---|---|---|---|
| ASFV-p72F | CGAAGGGAATGGATACTGA | 563 | Amplification of p72 |
| ASFV-p72R | TGTACCCGGCACAAAGA | ||
| ASFV-p72qF | ACGTTCGCTGCGTATCATTT | 112 | qPCR for detection of p72 |
| ASFV-p72qR | GAGGTATCGGTGGAGGGAAC | ||
| ASFV-probe | FAM- TGCACAAGCCGCACCAAAGCA-TAMRA | ||
| PCV2-CapF | TTACACGGATATTGTATTCCTGGTCG | 295 | Amplification of Cap |
| PCV2-CapR | GTGGGCTCCAGTGCTGTTATTCTA | ||
| PCV2-CapQF | AGTCTCAGCCACAGCTGATT | 128 | qPCR for detection of Cap |
| PCV2-CapQR | TCCTCCCGCCATACCAT | ||
| PCV2-probe | Cy5-AGCCCTTCTCCTACCACTCCCGCT-BHQ2 | ||
| PRV-gEF | GCGGCTGTTTGTGCTG | 413 | Amplification of gE |
| PRV-gER | CATAGTTGGGTCCATTCGT | ||
| PRV-gEqF | GCTCCTTCGTGATGACGTG | 131 | qPCR for detection of gE |
| PRV-gEqR | GTACACCGGAGAGAGCATGT | ||
| PRV-Probe | Texas Red-CTGCGTGCTGTGCTCCCGGC-BHQ2 | ||
| β-ActinqF | CCCTGGAGAAGAGCTACGAG | 175 | qPCR for detection of β-Actin [34] |
| β-ActinqR | AGGTCCTTCCTGATGTCCAC | ||
| β-Actin-probe | HEX-CGGCAACGAGCGCTTCCGGT-BHQ1 |