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. 2023 Feb 1;10(2):106. doi: 10.3390/vetsci10020106

Table 4.

Repeatability analysis of the multiplex real-time qPCR.

Plasmid Concentration
(Copies/μL)
Intra-Assay Inter-Assay
Mean S.D. CV (%) Mean S.D. CV (%)
pEasy-ASFV 107 15.36 0.132 0.86 15.21 0.058 0.39
106 18.19 0.078 0.43 18.12 0.012 0.06
105 21.44 0.083 0.39 21.57 0.073 0.34
104 25.47 0.276 1.08 25.05 0.493 1.96
pEasy-PCV2 107 15.53 0.104 0.67 15.47 0.048 0.31
106 18.8 0.139 0.74 18.88 0.063 0.33
105 20.85 0.237 1.14 21.47 0.380 1.73
104 24.76 0.297 1.20 25.04 0.203 0.81
pEasy-PRV 107 15.53 0.104 0.67 15.49 0.027 0.18
106 18.80 0.139 0.74 18.77 0.134 0.71
105 20.85 0.237 1.14 20.81 0.127 0.61
104 24.76 0.297 1.20 24.70 0.134 0.54