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. 2023 Feb 11;28(4):1725. doi: 10.3390/molecules28041725

Figure 5.

Figure 5

(A,B) Surface marker CD44 analyzed using flow cytometry in AGS after macrophage-conditioned medium treatment for 48 h. Data are presented as mean ± SEM for four independent experiments. Significance was calculated by one-way ANOVA analysis: *** p < 0.005. (C,D) p-STAT3 and STAT3 protein expression and quantification by Western blotting after treatment of AGS with 20 ng/mL IL-6 for 48 h. (E,F) OCT4 quantification by Western blotting after treatment of AGS with 20 ng/mL IL-6 for 48 h. (G,H) Surface marker CD44 analyzed using flow cytometry in AGS after treatment with 20 ng/mL IL-6 for 48 h. Data are presented as mean ± SEM for three independent experiments. Two-tailed Student’s t-test: * p < 0.05, ** p < 0.01 and *** p < 0.005.