FIG. 2.

Viability of the ypfP::cat mutant in the presence of phosphate-saline buffer. The mutant (3 × 10⁶ cells) and wild type (3 × 10⁶ cells) were maintained in 0.05 M phosphate-saline buffer (pH 7.2) (PSB) at 37°C for the indicated time. The numbers of CFU were determined from LB agar plates and are reported as percent survivors (viability). For comparison, the wild type and mutant were also maintained in 0.05 M phosphate buffer (pH 7.2) (PB). In addition, 0.1% d-glucose (Glc) added to PSB containing the parent and mutant are compared.