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. 2023 Feb 16;24(4):4007. doi: 10.3390/ijms24044007

Figure 1.

Figure 1

A schematic illustration of ant-human CD44 mAbs production. A BALB/c mouse was intraperitoneally immunized with CHO/CD44v3-10 cells. Hybridomas were produced by the fusion of the splenocytes and P3U1 cells. Then, the screening was performed by flow cytometry using parental CHO-K1 and CHO/CD44v3-10 cells. After cloning and additional screening, a clone C44Mab-9 (IgG1, kappa) was established. Finally, the binding epitopes were determined by enzyme-linked immunosorbent assay (ELISA) using peptides that cover the extracellular domain of CD44v3-10.