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. 2023 Jan 18;13(2):150. doi: 10.3390/metabo13020150

Figure 1.

Figure 1

The fraction of propidium iodide (PI) and Syto9 stained cells in an unquenched E. coli culture and a cold methanol-quenched (−30 °C) E. coli culture with and without subsequent washing in cold methanol (−30 °C), as estimated by fluorescence microscopy imagining. The average ± SD of n = 5 technical replicates is presented. More than 50 cells were counted per technical replicate.