Figure 3.

Detection of melanin in parental and mutant S. apiospermum strains. (a) Macroscopic aspect of parental and mutant strains after nine days at 37 °C on PDA medium. WT-TRC indicates the WT strain grown on a PDA plate supplemented with 50 µg/mL tricyclazole as a specific inhibitor of melanin biosynthesis. (b) Melanin quantity per million conidia, extrapolated from a standard curve of synthetic melanin, determined by EPR (Graph representative of at least three independent measurements). T: transformant derived from the WT strain; M1 and M2: transformants derived from the ΔKU70 strain.