Table 2.
qPCR validation of RNA-seq results on selected genes.
| Gene CDS ID | Description | M1 | ||
|---|---|---|---|---|
| RNA-seq | qPCR | |||
| Log2FC | p | Log2FC | ||
| SAPIO_CDS8655 | PIG1 | −3.7890 | 4.62 × 10−12 | −15.09 |
| SAPIO_CDS5047 | Scytalone deshydratase (SCD) | −6.1180 | 1.08 × 10−36 | −4.825 |
| SAPIO_CDS8657 | Tetrahydroxynaphthalene reductase (THNR) | −1.7735 | 0.2442 | −2.533 |
| SAPIO_CDS8658 | Conidial yellow pigment biosynthesis polyketide synthase (PKS) | −1.7835 | 0.0011 | −2.133 |
| SAPIO_CDS8659 | iron transport multicopper oxidase FET3 (ABR1) | −0.8088 | 0.1608 | −0.881 |
| SAPIO_CDS6958 | Conidiation specific protein-6 (CON6) | −3.1829 | 0.0298 | −3.343 |
| SAPIO_CDS1830 | Thioredoxin reductase | 1.5835 | 0.2752 | 0.5800 |
| SAPIO_CDS8079 | Peptidase C2, calpain family | −5.2099 | 1.60 × 10−10 | −5.305 |
Log2FC: mean values from four independent biological replicates. Statistically significant differential gene expression values (log2FC) are shown in bold. M1: transformant derived from the ΔKU70 strain.