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. Author manuscript; available in PMC: 2023 Feb 25.
Published in final edited form as: Cell Rep. 2022 Mar 29;38(13):110577. doi: 10.1016/j.celrep.2022.110577

Figure 6. MAPK regulation of AMPAR transport requires the scaffold proteins.

Figure 6.

(A) Kymographs of GLR-1::GFP transport in the AVA processes of transgenic control worms, unc-16(lf) and jip-1(lf), and transgenic control and mutant worms that expressed JKK-1::JNK-1::TagRFP-T gain-of-function chimera (JKK-1::JNK-1(gf)). All strains carried the glr-1(ky176) deletion mutation.

(B) Total GLR-1::GFP transport events in control without (n = 13) or with (n = 8) JKK-1:JNK-1(gf); unc-16(lf) without (n = 9) or with (n = 9) JKK-1:JNK-1(gf); and jip-1(lf) without (n = 9) or with (n = 5) JKK-1::JNK-1(gf). Data for control without JKK-1::JNK-1(gf) same as that shown in Figure 4F. ***p < 0.001.

(C) Kymographs of mCherry::UNC-16 transport in transgenic control worms, and jnk-1(lf) and jkk-1(lf) mutants. All strains carried the unc-16(e109) mutation.

(D) Total mCherry::UNC-16 transport events in control (n = 9), jnk-1(lf) (n = 10), and jkk-1(lf) (n = 10). Significantly different from control, *p < 0.05 and ***p < 0.001.

(E) Kymographs showing JKK-1::JNK-1::TagRFP-T and GLR-1::GFP co-transport events (arrowheads) in the AVA processes.

(F) Kymographs showing JKK-1::JNK-1::TagRFP-T transport in the AVA processes of transgenic control worms, and unc-16(lf) and jip-1(lf) mutants.

(G) Total JKK-1::JNK-1::TagRFP-T transport events in control (n = 10), unc-16(lf) (n = 10), and jip-1(lf) (n = 9). *Significantly different from control, p < 0.01; n.s., not significant.

(H) Three examples of single-plane confocal images of GLR-1::GFP, JKK-1::JNK-1::TagRFP-T, and the merge of both channels.

Scale bars, 5 μm. Error bars represent SEM. All statistics in this figure used a one-way ANOVA with Dunnett’s multiple testing as described in STAR Methods. See also Tables S1 and S2.