Table 2.
Complete Box–Behnken design. A: total lipid concentration; B: pH of the dispersion media; C: molar percentage of cholesterol; D: drug-to-lipid molar ratio; EE: encapsulation efficiency; F1: Factor 1; F2: Factor 2; F3: Factor 3; F4: Factor 4; PDI: polydispersity index; R1: Response 1; R2: Response 2; R3: Response 3; R4: Response 4; R5: Response 5.
| F1 | F2 | F3 | F4 | R1 | R2 | R3 | R4 | R5 | |
|---|---|---|---|---|---|---|---|---|---|
| Run | A (mg/mL) |
B | C (%) |
D (%) |
Liposome Size (nm)± SD | PDI ± SD | EE (%) | Zeta Potential (mV) |
PDI after 1 Month ± SD |
| 1 | 10 | 6.5 | 20 | 8 | 98 ± 1.3 | 0.12 ± 0.01 | 95 | −26 | 0.12 ± 0.01 |
| 2 | 10 | 4.75 | 0 | 5 | 94 ± 0.4 | 0.33 ± 0.01 | 96.5 | −28 | 0.55 ± 0.12 |
| 3 | 10 | 3 | 20 | 8 | 200 ± 1.6 | 0.30 ± 0.02 | 78 | −17 | 0.25 ± 0.06 |
| 4 | 10 | 4.75 | 20 | 11 | 102 ± 0.4 | 0.16 ± 0.00 | 87.3 | −26 | 0.28 ± 0.01 |
| 5 | 10 | 3 | 10 | 11 | 230 ± 10.3 | 0.40 ± 0.05 | 80.3 | −15 | 0.50 ± 0.05 |
| 6 | 10 | 6.5 | 10 | 5 | 85 ± 0.60 | 0.25 ± 0.01 | 88.5 | −24 | 0.38 ± 0.02 |
| 7 | 15 | 4.75 | 10 | 5 | 105 ± 1.5 | 0.27 ± 0.00 | 86 | −24 | 0.38 ± 0.01 |
| 8 | 10 | 3 | 10 | 5 | 165 ± 1.6 | 0.28 ± 0.01 | 91.5 | −15 | 0.26 ± 0.01 |
| 9 | 10 | 6.5 | 10 | 11 | 144 ± 1.4 | 0.26 ± 0.02 | 94 | −21.5 | 0.25 ± 0.02 |
| 10 | 15 | 6.5 | 10 | 8 | 120 ± 0.5 | 0.26 ± 0.00 | 94 | −26.5 | 0.38 ± 0.01 |
| 11 | 10 | 4.75 | 10 | 8 | 135 ± 9.5 | 0.22 ± 0.01 | 90 | −22.9 | 0.38 ± 0.01 |
| 12 | 10 | 4.75 | 10 | 8 | 112 ± 1.9 | 0.28 ± 0.03 | 91 | −26 | 0.30 ± 0.03 |
| 13 | 5 | 4.75 | 10 | 5 | 120 ± 1.0 | 0.17 ± 0.05 | 82.5 | −26 | 0.30 ± 0.03 |
| 14 | 5 | 3 | 10 | 8 | 220 ± 3.5 | 0.40 ± 0.03 | 69 | −13 | 0.50 ± 0.03 |
| 15 | 10 | 4.75 | 0 | 11 | 170 ± 2.7 | 0.40 ± 0.05 | 95.5 | −18 | 0.60 ± 0.03 |
| 16 | 15 | 3 | 10 | 8 | 174 ± 2.3 | 0.25 ± 0.02 | 96 | −16 | 0.33 ± 0.02 |
| 17 | 5 | 4.75 | 10 | 11 | 119 ± 0.3 | 0.22 ± 0.01 | 77.5 | −25 | 0.29 ± 0.03 |
| 18 | 10 | 6.5 | 0 | 8 | 160 ± 1.1 | 0.32 ± 0.02 | 96 | −19 | 0.30 ± 0.01 |
| 19 | 15 | 4.75 | 10 | 11 | 138 ± 0.2 | 0.21 ± 0.01 | 95 | −22 | 0.21 ± 0.01 |
| 20 | 15 | 4.75 | 0 | 8 | 167 ± 2.3 | 0.36 ± 0.01 | 95.5 | −21.5 | 0.50 ± 0.05 |
| 21 | 5 | 4.75 | 0 | 8 | 180 ± 1.7 | 0.39 ± 0.02 | 93 | −18.5 | 0.50 ± 0.02 |
| 22 | 15 | 4.75 | 20 | 8 | 109 ± 0.4 | 0.20 ± 0.01 | 92.5 | −24 | 0.21 ± 0.01 |
| 23 | 5 | 4.75 | 20 | 8 | 110 ± 0.3 | 0.19 ± 0.01 | 81 | −24 | 0.21 ± 0.01 |
| 24 | 5 | 6.5 | 10 | 8 | 120 ± 0.5 | 0.25 ± 0.00 | 92 | −25 | 0.26 ± 0.01 |
| 25 | 10 | 4.75 | 20 | 5 | 100 ± 1.2 | 0.16 ± 0.01 | 93.5 | −24 | 0.20 ± 0.02 |
| 26 | 10 | 4.75 | 10 | 8 | 116 ± 1.1 | 0.22 ± 0.01 | 94 | −24 | 0.28 ± 0.02 |
| 27 | 10 | 3 | 0 | 8 | 210 ± 11.7 | 0.45 ± 0.07 | 94.4 | −12.5 | 0.60 ± 0.04 |
| 28 | 10 | 4.75 | 10 | 8 | 118 ± 2.4 | 0.27 ± 0.04 | 93 | −22 | 0.34 ± 0.04 |
| 29 | 10 | 4.75 | 10 | 8 | 122 ± 8.3 | 0.22 ± 0.01 | 92 | −23 | 0.30 ± 0.02 |