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. 2023 Jan 19;15(2):339. doi: 10.3390/pharmaceutics15020339

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Quantitative determination of extracellular matrix mineralization with hMSCs cultures in the presence of PA, SrPhy or ZnPhy at 5 and 10 µg/mL after 21 days. The results for each concentration were statistically compared relative to the control group (osteogenic-DMEM) (ANOVA, *** p < 0.001, Tukey’s test), and between the two concentrations tested in each compound (ANOVA, # p < 0.001, Tukey’s test). The results are represented as the mean AzR concentration (mM) ± SD (n = 3). (B) Representative microscopic images (4×) of the AzR staining after 21 days of culture. Scale bars correspond to 500 µm.