Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Detection of neutralizing antibodies in the serum samples of immunized calves at day 0, 21, 35, and 49 after immunization. Inactivated serum samples were ratio diluted and pre-incubated with 200 TCID50 BVDV XZ02 for 1 h. Serum samples were subsequently added to cells in 96-well plates and incubated for 72 h. Rabbit anti-E2 antibody and FITC-conjugated goat anti-rabbit IgG were employed as the primary and secondary antibody. n = 5, * p < 0.05, and ** p < 0.01.
