Figure 1.

T-cell infiltration marker and PD-L1 in pancreatic cancer. (A) Image of a whole section of anti-CD3-stained tissue displaying the regions of tumor and the invasive margin. The immune hotspots are shown with arrows. (B) A hotspot in the tumor shows numerous positive T cells and fewer tumor cells. (C) The corresponding image analysis shows the counted cells. (D) Representative pictures for low and high CD3, CD4, CD8, FoxP3, and PD-L1 immunostaining (brown) in PC tumors. Original magnification, 20×; scale bars, 100 µm. Enlarged images on the right panel are shown for the areas outlined by black squares. Scale bars show 25 µm in length.