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. 2023 Feb 15;24(4):3929. doi: 10.3390/ijms24043929

Table 1.

Chromatographic gradient.

Time (Minutes) %C Flow mL/min
0 2 0.250
2.5 2 0.250
3 80 0.250
7 80 0.250
8 2 0.250
15 2 0.250