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. 2023 Feb 5;24(4):3165. doi: 10.3390/ijms24043165

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Panoramic micrographs of intact nuclei isolated from the adult rat brain cortex processed for MGL-, ABHD12- and COX2-immunofluorescence (A–C) combined with NeuN/Fox-3-immunofluorescence (D–F) and Hoechst’s chromatin staining (G–I). Every NeuN-positive nucleus exhibited strong immunoreactivity for MGL, ABHD12 and COX2 (filled arrowheads), whereas NeuN-negative nuclei (empty arrowheads) displayed clear positive staining only for COX2. All micrographs were acquired in grayscale and pseudo colored. Scale bar = 20 µm in I (applies to (A–I)).