Table 1.
Target | Dilution | Host/Clonality | Isotype | Antigen | Reference | |
---|---|---|---|---|---|---|
IF | WB | |||||
ABHD12 | 1:200 | 1:2500 | Goat polyclonal | Affinity purified (ns) | C-REFLGKSEPEHQH peptide | Sigma-Aldrich, SAB2500016 |
COX2 | 1:200 | 1:2500 | Goat polyclonal | IgG | Peptide located at the C-terminus of human COX2 | Sta Cruz Biotech., sc-1745 |
DGLα | 1:250 | 1:1000 | Rabbit polyclonal | Affinity purified (ns) | Peptide corresponding to the 42 amino acids of the C-termninal end of mouse DGLα | Frontier Science, DGLα-Rb-Af380-1 |
FAAH | − | 1:1000 | Goat polyclonal | IgG | Peptide mapping near the N-terminus of human FAAH | Sta Cruz Biotech., sc-26427 |
GRP78/BiP | − | 1:2000 | Rabbit polyclonal | IgG | Peptide corresponding the 55 C-terminal aas of GRP78/BiP | Abcam, ab21685 |
Histone H3 | − | 1:500 | Rabbit polyclonal | IgG | Peptide corresponding to aas 1–20 of histone H3 | Chemicon, 382157 |
MGL | 1:200 | 1:1000 | Goat polyclonal | IgG | Peptide corresponding to aas 17–29 of human MGL | Abcam, ab77398 |
Na+/K+ ATPase | − | 1:5000 | Mouse monoclonal | IgG1 | α1 subunit of Na+/K+ -ATPase purified from lamb liver | Sigma-Aldrich, A-277 |
NeuN/Fox-3 | 1:1000 | 1:2000 | Mouse monoclonal | IgG1 | Nuclei purified from mouse brain | Chemicon, MAB377 |
NPCx | 1:4000 | 1:5000 | Mouse monoclonal | IgG1 | Protein mixture of the nuclear pore complexes | Abcam, ab24609 |
Thy1/CD90 | − | 1:6000 | Rabbit monoclonal | IgG | Epitope located near the N-terminus of human Thy1/CD90 | Abcam, ab92574 |
β-tubulin | − | 1:1000 | Mouse monoclonal | IgG1 | Epitope between aas 281–446 at the C-terminus of β-tubulin | Sigma-Aldrich, T4026, |
ns, not specified by the vendor.