Figure 4.

Multivariate analyses of metabolites of mealy and juicy ripening nectarines after cold storage. Partial Least Squares Regression Discriminant Analysis (PLS-DA) for S3 and S4 stages and score plot of mealy (A) and juicy (B) varieties. The identified metabolites were employed as predictor variables and the varieties as response variables. Heatmap representation based on top 25 metabolites identified by PLS-DA VIP of S3 and S4 stages from the mealy (C) and the juicy (D) varieties. The columns represent biological replicates for each treatment. The similarity measure used to group the different characteristics was based on Euclidean distance and Ward’s linkage from three biological replicates (n = 3). Pathway analysis of S3 and S4 stages from the mealy (E) and the juicy (F) varieties. Perturbed metabolic pathways and their KEGG entries are highlighted in grey (G). Three biological replicates (n = 3) and the Arabidopsis thaliana pathway library were used for this analysis. Volcano plots showing differentially accumulated metabolites (DAMs) from mealy (H) and juicy (I) varieties.