Table 2.
Association between PD-L1 (+/-) expressions in tumor microenvironment cells and CD4 and CD8 tumor-infiltrating lymphocytes.
| Nb. | Morphological Features | Infiltrating Immune Cells | X2 Score |
p-Value | Melanoma Tumor Cells | X2 Score |
p-Value | ||
|---|---|---|---|---|---|---|---|---|---|
| PD-L1 (-) n (%) |
PD-L1 (+) n (%) |
PD-L1 (-) n (%) |
PD-L1 (+) n (%) |
||||||
| 1. |
Morphologic type LMM SSM SSM with VGN NM |
2 (8.33%) 7 (29.17%) 6 (25%) 9 (37.50%) |
1 (2.70%) 14 (37.84%) 14 (37.84%) 8 (21.62%) |
3.305 |
0.332 |
3 (9.09%) 8 (24.24%) 13 (39.39%) 9 (27.27%) |
0 (0%) 13 (46.43%) 7 (25%) 8 (28.57%) |
5.678 |
0.144 |
| 2. |
TILs evaluation Absent Brisk Non-brisk |
8 (33.33%) 3 (12.50%) 13 (54.17%) |
2 (5.41%) 8 (21.62%) 27 (72.97%) |
8.383 * |
0.020 |
7 (21.21%) 4 (12.12%) 22 (66.67%) |
3 (10.71%) 7 (25%) 18 (64.29%) |
2.425 |
0.287 |
| 3. |
CD8 TIL’s Negative <5% 5–50% >50% |
9(37.50%) 1(4.17%) 14(58.33%) 0 (0.00%) |
2 (5.41%) 1 (2.70%) 32 (69.57%) 2 (5.41%) |
11.238 ** |
0.003 |
7 (21.21%) 0(0.00%) 25 (75.76%) 1 (50.00%) |
4 (14.29%) 2 (7.14%) 21 (75.00%) 1 (50.00%) |
2.775 |
0.514 |
| 4. |
CD4 TIL’s Negative <5% 5–50% >50 |
6 (25.00%) 1 (4.17%) 17 (70.83%) 0 (0.00%) |
0 (0.00%) 0 (0.00%) 36 (97.30%) 1 (2.70%) |
12.614 ** |
0.001 |
3 (9.09%) 0 (0.00%) 29 (87.88%) 1 (3.03%) |
3 (10.71%) 1 (3.57%) 24 (85.71%) 0 (0.00%) |
2.076 |
0.906 |
PD-L1- Programmed cell death ligand-1; TILs- tumor-infiltrating lymphocytes; LMM- lentigo maligna melanoma; SSM- Superficial spreading melanoma; SSM-VGN- superficial spreading melanoma with vertical growth nodule; NM- nodular melanoma; * p < 0.05 and ** p ≤ 0.001 represent statistically significant differences between cases made by Pearson Chi-Square with Cramer’s V test for the association between categorical variables, Mann-Whitney U Test and Kruskal-Wallis H Test for Continuous variables (X2 Score).