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. 2001 Oct;183(20):5813–5825. doi: 10.1128/JB.183.20.5813-5825.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 1 — Effects of VirB11 Walker A mutations on accumulation of T-pilus proteins and other VirB proteins in exocellular (A) and cellular (B) fractions obtained as described in the text. Strains: ΔB11(B11), strain PC1011(pSRB1) expressing virB11 from an IncP replicon; ΔB11, strain PC1011; ΔB11(ΔGKT), PC1011(pPCB7112) expressing virB11ΔGKT174-176; A348(ΔGKT), A348(pPCB7112) coexpressing virB11 and virB11ΔGKT174-176; ΔB11(K/Q), PC1011(pPCB7113) expressing virB11K175Q; A348(K/Q), A348(pPCB7113) coexpressing virB11 and virB11K175Q. M, molecular mass markers, with sizes in kilodaltons indicated on the right. The blots were developed with antisera to the VirB proteins listed on the left.