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Sucrose density gradient distribution profiles of T pili from various virB11 mutant strains. Exocellular proteins from the strains listed on the left were centrifuged through identically prepared sucrose density gradients, and the fractions were analyzed for the presence of VirB2 pilin. Strains: ΔB11(B11), PC1011(pXZB100); ΔB11(7), PC1011(pXZB107); A348(7), A348(pXZB107); A348(3), A348(pXZB103); ΔB2(B2C64S), PC1002(pVSB10) that synthesizes mutant pilin (53).
