FIG. 6.

Effects of IncQ plasmid on accumulation of VirB proteins and T-pilus proteins. (A) VirB and VirE2 protein levels in total-cell extracts of A348 and A348(pML122), with VirB proteins listed on the left of each immunoblot. M, molecular mass markers, with sizes in kilodaltons indicated on the right. (B) VirB2 pilin levels in exocellular fractions from A348 and merodiploid strains expressing the dominant alleles 1 through 12 and pML122ΔKm (designated pML). Also shown are the pilin levels in PC1011 (designated Δ11) expressing virB11 (B11; from plasmid pXZB100) and class III alleles.