TABLE 2.

Phenotypes of virB11 dominant alleles

Allele or strain type	pXZB1xxa plasmids or strain	Mutation	PC1011(pXZB1xx) strains			A348(pXZB1xx) merodiploid strains
			T-DNA transferb	pML122ΔKm mobilization (Tcs/donor)c	T-pilus productiond	T-DNA transferb	pML122ΔKm mobilization (Tcs/donor)c	T-pilus productiond
Class III (dominant	pXZB102	I265T	+++	8.5 × 10−4	−	±	6 × 10−3	+++
functional)	pXZB105	L75F	+++	1.6 × 10−3	++	+	3.6 × 10−3	+++
	pXZB106	Q135E	+++	1 × 10−3	++	±	1.6 × 10−4	+++
	pXZB107	V258G	+++	2.5 × 10−5	+	+	6 × 10−3	+++
	pXZB110	V116I	+++	1.4 × 10−3	+++	+	2.5 × 10−3	+++
	pXZB111	V49A/E256G	+++	2 × 10−4	++	+	4.5 × 10−3	+++
	pXZB112	N73Y/T98I	+++	3.3 × 10−4	++	+	3.5 × 10−3	+++
Class II (dominant	pXZB101	I88T/I103T	−	2.4 × 10−7	−	+	5 × 10−3	+++
nonfunctional)	pXZB103	H269R	−	<10−7	−	+	2.5 × 10−4	+++
	pXZB108	L11P/D56G/E335G	−	5 × 10−7	−	+	4.2 × 10−3	+++
	pXZB109	I103T/M301L	−e	<10−7	−	+	8 × 10−3	+++
Control	A348	WTg	NAf	NA	NA	+++	3.7 × 10−3	+++
	PC1011	ΔvirB11	−	<10−7	−	NA	NA	NA
	PC1011(pXZB100)	PvirB::virB11	+++	4.5 × 10−3	+++	NA	NA	NA
	PC1011(pPCB7111)	Plac::virB11	+++	4.1 × 10−4	++	NA	NA	NA

^aUnderlined number denotes allele, as originally named in Zhou et al. (69). 

^bMonitored by virulence assays on Kalanchoe wound sites. +++, wild-type transfer; ±, barely detectable transfer; −, no transfer 

^cData are the means of three trials from a single experiment. Three independent experiments yielded similar results. 

^d+++ abundant pilus production, − no detectable pilus proteins in exocellular fraction. 

^ePC1011(pXZB109) exported VirE2 but not DNA substrates, as determined by mixed-infection assays. 

^fNA, not applicable. 

^gWT, wild type.