TABLE 3.
Phenotypes of the virB11.i4 alleles
| PC1011(pJC8xxx) or control strain | i4 insertion sitea | Dominanceb | T-DNA transferc | Transfer by mixed infectionc
|
pML122ΔKm mobilization (Tcs/input donor)cd | T-pilus productiona | |
|---|---|---|---|---|---|---|---|
| VirE2 | T-strand/D2 | ||||||
| pJC8018 | L18 | − | + | − | − | <10−7 | − |
| pJC8056 | D56 | − | − | − | − | <10−7 | − |
| pJC8075 | L75 | + | − | ++ | − | 9.5 × 10−6 | − |
| pJC8088 | L88 | − | − | − | − | 5 × 10−7 | − |
| pJC8115 | E115 | − | − | − | − | 2 × 10−7 | − |
| pJC8135 | Q135 | − | +++ | NA | NA | 2 × 10−3 | +++ |
| pJC8168 | C168 | − | − | ++ | − | 1.5 × 10−5 | − |
| pJC8196 | E196 | +++ | − | − | − | <10−7 | − |
| pJC8217 | G217 | − | +++ | NA | NA | 5.5 × 10−4 | +++ |
| pJC8237 | P237 | − | +++ | NA | NA | 1.2 × 10−3 | +++ |
| pJC8260 | G260 | − | − | − | − | 1 × 10−7 | − |
| pJC8281 | F281 | − | − | − | − | 1.3 × 10−7 | − |
| pJC8302 | L302 | − | − | ++ | − | 9.3 × 10−6 | − |
| pJC8323 | E323 | + | − | − | − | 1.5 × 10−7 | − |
| A348 | WT | +++ | NA | NA | 3 × 10−3 | +++ | |
| PC1011 | ΔvirB11 | − | − | − | − | <10−7 | − |
| PC1011(pXZB100) | (PvirB::virB11) | − | +++ | NA | NA | 3 × 10−3 | +++ |
a
Indicated residue immediately precedes i4 insertion.
b
Virulence of the virB11/virB11.i4 merodiploid strain is strongly (+++) or weakly (+) attenuated or not attenuated (−).
c
See footnotes to Table 1 for meaning of symbols.
d
Data are the means of three trials from a single experiments. Three independent experiments yielded similar results.