Figure 2.
Effect of broccoli extracts on mitochondrial function of HepG2 cells. (A) Representative image of oxygen consumption rate (OCR) measurement in HepG2 cells treated with vehicle (CTL) or leaf (E1—green), stalk (E2—blue), or inflorescence (E3—red) Brassica extracts for 48 h. Several OCR parameters were evaluated: (B) cellular basal OCR; (C) maximal OCR; (D) ATP-production-linked OCR; (E) proton-leak-linked OCR; (F) non-mitochondrial OCR; and (G) basal extracellular acidification (ECAR). Data are the mean ± SEM of 3 independent experiments, and the results are expressed as pmol O2/min/cell mass for OCR and mpH/min/cell mass for ECAR. The data obtained with BBP extracts were compared to CTL using a one-way ANOVA with Dunnett’s multiple comparison post-test.