Figure 5.

Effect of broccoli extracts on the activity of antioxidant enzyme catalase under supra-physiological concentrations of fatty acids (FA) in human HepG2 cells. HepG2 cells were (A–C) co-incubated or (D–F) pre-incubated with BBP extracts ((A,D) leaves—E1, (B) and (E) stalks—E2, or (C,F) inflorescences—E3) and then subjected to supraphysiological concentrations of free fatty acids. Catalase activity was assessed in HepG2 cells through the capacity to decompose H₂O₂. Data are the mean ± SEM of four different assays performed, and the results were normalized on the control condition (CTL = 100%). The data obtained with BBP extracts were compared to CTL using a one-way ANOVA with Dunnett’s multiple comparison post-test. Significant differences between the indicated conditions are marked by * (p < 0.05).