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. 2023 Feb 7;12(2):269. doi: 10.3390/pathogens12020269

Figure 2.

Figure 2

Binding capacity of soil minerals to WTD-CWD prions. Identical amounts of 10% infected deer brain homogenate (BH) were incubated with water (control) and 15 mg/mL suspension of soil minerals quartz (Qz), montmorillonite (Mte), Illite (Ill) and soils Leptosol (Lep), Luvisol (Luv) and Chernozem (Cher) overnight at 4 °C. Samples were fractionated through a 1M sucrose cushion to separate bound prions from unbound; pellet (p.; bound prions) and supernatant (sup; unbound prions) were analyzed by Western blot using mAb Bar224.