Figure 3.

Leachate obtained at the first (A) and second (B) week and at weeks 1–4 (C,D) from columns with quartz (Qz), illite (Ill) with Mte (column experiment 1): Qz—pure quartz; Ill—illite (30%) + quartz; Mte—montmorillonite (30%) + quartz (70%). Approximately 5 mL of leachate was obtained from each column every week; 50 μL of leachate were boiled with 5 × Laemmlii buffer and analyzed by Western blot using mAb Bar224 (1:10,000). Initial amount of BH applied for the columns was used as a positive control (first lanes on each panel).