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. 2023 Feb 7;12(2):269. doi: 10.3390/pathogens12020269

Figure 4.

Figure 4

Leachate obtained at the first (A) and second (B) week from all 9 columns (column experiment 2): Qz—quartz; Ill—illite (50%) + quartz; Mte—montmorillonite (50%) + quartz; Luv—northern Luvisol; horizons LFH and Ae; Brun—mountain Brunisol; horizons LFH and Bf; Ch—Chernozems. Approximately 5 mL of leachate was obtained from each column; 50 μL of leachate was boiled at 100 °C with 5 × Laemmlii buffer and analyzed by Western blot using mAb Bar224 (1:10,000). Initial amount of BH applied for the columns was used as a positive control (first lanes on each panel).