Figure 5.
PMCA seeding activity of leachates from the columns. Second round of PMCA: the amplification of PrPCWD in leachates obtained at the third week with quartz (Qz), illite (Ill), Brunisolic Bf horizon (Br), Luvisolic Ae horizon (Luv) and southern Chernozem (Ch) (experiment 2) columns. CWD-PrP (−3; −4; −5)—amplification control; Qz, Br, Ch, Ill, Luv—samples from column; NBH—uninfected brain homogenate diluted 1:10 in the PrPC substrate; no PMCA control. The same samples were stored at 37 °C without sonication. The samples were PK digested (50 µg/µL) and analyzed by WB using mAb Bar224 (1:10,000).