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. 2023 Jan 27;15(2):109. doi: 10.3390/toxins15020109

Figure 3.

Figure 3

Enzymatic activity on different substrates with increasing amounts of detergent. LiRecDT1 wild type toxin (10 µg) was added to (A) SM, (B) LysoPC, and (C) PC substrate (250 µM) solubilized in detergent Triton-X-100 (0.1%, 0.5%, and 1%). PLD activity was measured using the Amplex Red kit (Invitrogen). Control: incubation of Am plex Red reagent mixture without the enzyme. After incubation (30 min, 37 °C), fluorescence was measured by fluorimeter, excitation 540 nm, and emission 570 nm (Tecan Infinite M200). The values represent average ± SD of three independent experiments carried out in triplicate (**** p < 0.001 and “ns”: non-significant).