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. 2023 Jan 27;15(2):109. doi: 10.3390/toxins15020109

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Analysis of the cleavage activity of Brown spider recombinant phospholipase-D (LiRecDT1) on different phospholipid substrates, studied by using NMR spectroscopy. (A) In situ NMR histograms of SM (16:0), SM (6:0), PC (16:0), LPC (16:0), and LPC (6:0) (1 mM) after recombinant phospholipase-D treatment. (B) Product generation plot during phospholipase-D (1 μg LiRecDT1) incubation with different phospholipids substrates in 100 mM Tris-HCl pH: 7.4, 100 mM NaCl, and 5 mM MgCl₂ and 0.01% Triton X-100 (20 °C). Product formation was evaluated by NMR (Bruker AVANCE III HD spectrometer) every 10 min for 4 h. [S]: substrate concentration. [P]: product concentration.