Table 1.
Kinetics parameters of the Brown spider recombinant phospholipase-D (LiRecDT1).
| Sphingomyelins V0 (μM.min−1) |
Phosphatidylcholines V0 (μM.min−1) |
||
|---|---|---|---|
| SM 24:0 | 0.618 | PC 16:0 | 0.212 |
| SM 18:0 | 2.257 | LPC 24:0 | 0.287 |
| SM 16:0 | 2.537 | LPC 18:0 | 1.199 |
| SM 12:0 | 2.933 | LPC 16:0 | 1.906 |
| SM 06:0 | 3.493 | LPC 12:0 | 1.805 |
| SM 02:0 | 1.124 | LPC 06:0 | 0.238 |
| LSM 0:0 | 0.206 | ||
Reaction rates (µM.min−1) of LiRecDT1 with various substrates: reported values are rates of reaction for the activity of LiRecDT1 over 5 mM of different substrates (30 min).