Figure 7.

Schematic diagram depicting the basic steps of CLIA for the detection of anti-VZV antibodies. (1) Magnetic microspheres are coated with the VZV antigen (e.g., VZV gE) and incubated overnight at 4 °C with shaking. (2) After washing, microspheres are blocked with 10% BSA and incubated for 2 h at 37 °C with shaking. (3) After washing, diluted serum samples are added to the coated microspheres and incubated for 2 h at 37 °C with shaking. (4) After washing, anti-human IgG conjugated with acridinium is added and incubated for 1 h at room temperature (RT) with shaking. (5) After washing, the magnetic microspheres are resuspended with PBS and the chemiluminescent signal is detected with a chemical luminescence immune analyzer.