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Melting temperatures obtained from nanoDSF protein-chemical binding validation for the interaction of HSPB1 with TCDD at 5 nM (20%) (yellow), 15 nM (60%) (orange), and 25 nM (100%) (red). A control without TCDD was included (light blue). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).
