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. 2023 Feb 10;24(4):3598. doi: 10.3390/ijms24043598

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Circulating neutrophils over time after HI following NT and TH treatment in different samples. The proportion of neutrophils was quantified by flow cytometry using specific antibodies against the rat tissues. Gate strategy (a) was performed considering granulocyte, single, and live cells, following all CD45 positive cells, and considering the percentage of all positive RP-1 respect CD11_b/c cells. The percentage of all RP-1⁺/CD11_b/c⁺ neutrophils in the brain (b), blood (c), bone marrow (d), and spleen (e) at the indicated time points after HI following NT or TH compared to the sham sample. Sham (white column), HI (grey with cross-line column), HI/NT (grey column), and HI/TH (black column). Data of ten biological replicates (n = 10) per time point and condition (see Section 4). Two-way ANOVA was used with a * p < 0.05. Data are expressed as mean ± SD.